biotinylated secondary antibody (Vector Laboratories)

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Vector Laboratories biotinylated secondary antibody
Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 9804 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+secondary+antibodies/Biotinylated+Anti-Avidin+Antibody/pmc13099946-80-7-11
Average 96 stars, based on 9804 article reviews

biotinylated secondary antibody - by Bioz Stars, 2026-09

96/100 stars

Images

Binding Assay:

Article Title: High neuron-microglia interaction at the node of Ranvier predicts recovery in an inflammatory model of Multiple Sclerosis
Article Snippet: .. Binding of biotinylated secondary antibodies (Vector Laboratories) was visualized using the avidin-biotin horseradish peroxidase complex (Dako, Biotin Blocking System) and 3,3’-diamino-benzidine (DAB) (Vector Laboratories) as substrate. .. In parallel, primary antibodies were also detected using the ABC-alkaline phosphatase detection system (Vector Laboratories) with Vector Blue as the substrate.

Avidin-Biotin Assay:

Article Title: Nuclear hexokinase 2 couples hyperglycemia to MYC-driven glycolytic and stemness programs in bladder cancer.
Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against HK2, MYC, LDHA, or CD44 (all from Cell Signaling Technology, CST, USA). .. After washing, sections were incubated with the appropriate biotinylated secondary antibodies at room temperature for 1 h, followed by color development using an avidin–biotin complex (ABC) detection kit (Vector Laboratories, .. Schematic diagrams AR TI CL E IN P RE SS Schematic diagrams were created using Figdraw.

Article Title: Glial pathology networks reveal early olfactory vulnerability in post mortem human Alzheimer's disease
Article Snippet: .. After washing in TBS‐T, sections were incubated with biotinylated secondary antibodies (1:200, 1 h, RT), followed by the Avidin‐Biotin Complex (ABC reagent, Vector Laboratories, 2 h, RT). .. DAB substrate solution (Sigma‐Aldrich) was applied for 3 to 10 min until signal developed.

Blocking Assay:

Incubation:

Article Title: Lilii bulbus Exerts Anti-Seizure Effects by Modulating GABAergic Synapse Organization in the Pentylenetetrazol Kindling Model
Article Snippet: .. The tissues were then incubated with primary antibodies at 4 °C for 24 h. Subsequently, the sections were treated with biotinylated secondary antibodies (1:300; Vector Laboratories Inc., Newark, CA, USA) for a 2 h duration at room temperature. .. Following a TBS wash, an avidin–biotin complex solution was applied for 1 h. Representative images were captured utilizing an Olympus BX53 optical microscope (Olympus) Primary antibodies used in this study included glutamate decarboxylase 67 (GAD67; 1:1000, Millipore, Waltham, MA, USA), vesicular GABA transporter (VGAT; 1:1000, Thermo Scientific, Waltham, MA, USA), parvalbumin (PV; 1:1000, Synaptic Systems, Göttingen, Germany), and somatostatin (SOM; 1:1000, Abcam, Cambridge, UK).

Article Title: REST-AKT-SOX2 axis controls neuronal lineage state and Midkine-mediated communication in SHH-medulloblastoma
Article Snippet: Tissue sections of 5 μm thickness were prepared and deparaffinized using a Gemini AS Automated Stainer (Thermo Fisher Scientific). .. Following overnight incubation with primary antibodies at 4 °C, sections were treated with biotinylated secondary antibodies supplied with either the ABC kit or the MOM kit (Vector Laboratories, CA, USA). .. Signal detection was performed using the VECTASTAIN® Elite® ABC-HRP Kit, Peroxidase (Vector Laboratories, Cat# PK-6101), according to the manufacturer’s protocol, and visualized with the DAB Peroxidase Substrate Kit (Vector Laboratories, Cat# SK-4100).

Article Title: Western diet suppresses canonical intestinal stem cells and reprograms c-Kit⁺ reserve stem cells via proinflammatory dysbiosis
Article Snippet: Sections were blocked with 4% goat serum (GS, Thermo Fisher Scientific, 16210064) in PBST for 1.5 hours at room temperature, then incubated overnight at 4°C with the following primary antibodies: β-catenin (Cell Signaling Technology, 8814); YAP (Cell Signaling Technology, 14074T); and Ki67 (BioLegend, 652402). .. Sections were washed three times in 2% GS in PBST, then incubated for 1.5 hours at room temperature with the following biotinylated secondary antibodies diluted in 2% GS in PBST: Anti-Rabbit (Vector Laboratories, BA-1000-1.5), and Anti-Rat (Vector Laboratories, BA-1000-1.5). .. Following three washes in 1% GS in PBST, sections were incubated with avidin-biotin complex (ABC) solution (Vector Laboratories, PK-6100) for 1 hour at room temperature in the dark.

Article Title: Altered AMPA receptor subunit configuration contributes to dysfunctional excitatory synaptic activity in BCNU rat-model of focal cortical dysplasia (FCD).
Article Snippet: Focal Cortical Dysplasia (FCD) is a leading cause of drug-resistant epilepsy, yet its molecular drivers remain unclear.. AMPA receptor subunit composition plays a key role in synaptic excitability and may be altered in FCD.. To investigate whether dysregulated expression of AMPAR subunits, specifically GluA1 and GluA4, contributes to the enhanced excitatory synaptic activity in FCD rat-model, BCNU (1,3-bis(2-chloroethyl)-1-nitrosourea) was administered in-utero to induce FCD in rats.

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